Running Genalex

GenAlEx offers a wide range of population genetic analysis options for the full spectrum of genetic markers within the Microsoft Excel environment on both PC and Macintosh computers.
When combined with its user-friendly interface, rich graphical outputs for data exploration and publication, tools for data manipulation and export options to many other software packages, GenAlEx offers an ideal launching pad for population genetic analysis

LINK: Download GenAlEx here.


GenAlEx offers analysis of codominant, haploid and binary genetic loci and DNA sequences.

Both frequency-based (F-statistics, heterozygosity, HWE, population assignment, relatedness) and distance-based (AMOVA, PCoA, Mantel tests, multivariate spatial autocorrelation) analyses are provided.

GenAlEx introduces exciting new features including calculation of new estimators of population structure: G'ST, G"ST, Jost’s Dest, and F'ST via AMOVA, Shannon Information analysis, linkage disequilibrium analysis for biallelic data, and heterogeneity tests for spatial autocorrelation analysis.

Data format for GenAlEx

Self-paced GenAlEx Tutorials

Several self-paced tutorials on population genetic analysis that employ hand calculations and exercises are offered within GenAlEx. These are drawn in part from the graduate workshops that have been offered (jointly and independently), around the world.

Visit the links below to download tutorials on:
    An Introduction to Frequency-Based Population Genetic Analysis:
    • scoring genetic markers,
    • >Allele Frequency,
    • Heterozygosity,
    • F-statistics,
    • Nei Genetic Distance,
    • Shannon Diversity Indices
    • Chi-square tests for Hardy-Weinberg Equilibrium
    Genetic Distance and AMOVA:
    • Haploid,
    • Codominant and Binary Genetic Distance,
    • AMOVA
    • F-statistics
    Spatial Genetic Analysis:
    • Principal Coordinate Analysis (PCoA),
    • Mantel Tests for Matrix Correspondence
    • Spatial Autocorrelation Analysis
    Advanced Frequency-Based Analysis:
    • DNA Profile Probability,
    • Probability of Identity,
    • Probability of Exclusion,
    • Population Assignment
    • Pairwise Relatedness
    Advanced Features Including Data Import and Export:
    - Working with DNA sequences,
    - importing and processing raw genotypic data,
    - exporting data from GenAlEx to other software.

    The Stats menu and how to customise the GenAlEx menu are also covered briefly.