MUSCLE is a multiple alignment program with accuracy and speed that are consistently better than CLUSTALW.

• Version: 3.8.1551
• Added: April, 2020
• Updated: February, 2023
• Link: http://www.drive5.com/muscle/

See versions of muscle which are available:

$ module avail muscle

Load a particular version into your environment and run it:

$ module load muscle/3.8.1551
$ muscle -version
MUSCLE v3.8.1551 by Robert C. Edgar

Notes from the sysadmin during installation:

$ cd /tmp
$ wget http://www.drive5.com/muscle/muscle_src_3.8.1551.tar.gz
$ mkdir muscle
$ tar xf muscle_src_3.8.1551.tar.gz -C muscle
$ cd muscle
$ sudo dnf install glibc-static libstdc++-static.x86_64
$ make
$ sudo mkdir -p /export/apps/muscle/3.8.1551
$ sudo cp muscle /export/apps/muscle/3.8.1551

If you get an error about inability to link against -lm you need to install glibc-static.