FastANI is developed for fast alignment-free computation of whole-genome Average Nucleotide Identity (ANI). ANI is defined as mean nucleotide identity of orthologous gene pairs shared between two microbial genomes. FastANI supports pairwise comparison of both complete and draft genome assemblies.

• Version: 1.3
• Added: May, 2020
• Link: https://github.com/ParBLiSS/FastANI

See versions of fastANI which are available:

$ module avail fastANI

Load one version into your environment and run it:

$ module load fastANI/1.3
$ fastANI

Notes from the sysadmin during installation:

$ cd /tmp
$ wget https://github.com/ParBLiSS/FastANI/releases/download/v1.3/fastANI-Linux64-v1.3.zip
$ unzip fastANI-Linux64-v1.3.zip
$ sudo mkdir -p /export/apps/fastANI/1.3/bin
$ sudo cp fastANI /export/apps/fastANI/1.3/bin