Depends on samtools and boost! Make sure those are installed first…

tar zxf tophat-2.0.3.tar.gz
cd tophat-2.0.3

./configure --prefix=/export/apps/tophat/2.0.3 --with-boost=/export/apps/boost/1.47.0 --with-bam=/export/apps/samtools/0.1.18
make -j4
sudo make install

Allow users to load support for tophat using the module system:

sudo mkdir -p /export/apps/modules/modulefiles/tophat

Create the module file, /export/apps/modules/modulefiles/tophat/2.0.3:

#%Module1.0
#####################################################################
##
## cufflinks Modulefile
## by Alan Orth, a.orth@cgiar.org
##
set version   2.0.3
set prefix    /export/apps/cufflinks/${version}
set exec_prefix   ${prefix}
set url "http://cufflinks.cbcb.umd.edu/"
set msg "This module adds cufflinks v$version to various paths\n\ncufflinks Official Site: $url\n"

proc ModulesHelp { } { 
  puts stderr "$msg"
}

module-whatis   "$msg"

conflict      cufflinks

prepend-path  PATH    ${exec_prefix}/bin
# cufflinks 1.0.3 was compiled against boost 1.47.0, make sure if can find boost!
prepend-path  LD_LIBRARY_PATH /export/apps/boost/1.47.0/lib

# cufflinks depends on samtools, load the samtools module as well
if { ![is-loaded samtools] } { 
    module load samtools
}

module load cufflinks/2.0.0