trinity-software
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trinity-software [2015/03/20 15:32] – [Usage] aorth | trinity-software [2017/08/22 12:20] – aorth | ||
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===== Information ===== | ===== Information ===== | ||
- | * Version: v2.0.3 | + | * Latest |
- | * Added: | + | * Added: |
+ | * Updated: August, 2017 | ||
* Link: https:// | * Link: https:// | ||
===== Usage ===== | ===== Usage ===== | ||
- | === See versions of trinity which are available | + | See versions of trinity which are available: |
- | < | + | |
- | **Note:** Trinity creates millions of small files -- which causes problems on the HPC storage server -- so you must direct output from these jobs to /// | + | < |
- | < | + | **Note:** |
+ | * You should use the **−−full_cleanup** option so that Trinity can clean up after itself by removing all the intermediary files it has created except for the final Trinity.fasta results file. | ||
+ | * Trinity creates millions of small files -- which causes problems on the HPC storage server -- so you must direct output from these jobs to /// | ||
#SBATCH -p highmem | #SBATCH -p highmem | ||
#SBATCH -J trinity | #SBATCH -J trinity | ||
- | #SBATCH -n 10 | + | #SBATCH -n 8 |
- | module load trinity/ | + | module load trinity/v2.4.0 |
- | module load bowtie/ | + | |
- | module load samtools/ | + | |
- | # cd to working | + | # create and change |
- | export WORKDIR=$SLURM_JOBID | + | export WORKDIR=/ |
+ | mkdir -p $WORKDIR | ||
cd $WORKDIR | cd $WORKDIR | ||
# match CPUs to amount requested in SBATCH options! | # match CPUs to amount requested in SBATCH options! | ||
- | Trinity --seqType fq --max_memory 10G --left fish_R1.fastq --right fish_R2.fastq --CPU 10 --output trinity-fish2</ | + | Trinity --seqType fq --max_memory 10G --left fish_R1.fastq --right fish_R2.fastq --CPU 8 --output trinity-fish2 |
===== Installation ====== | ===== Installation ====== | ||
Notes from the sysadmin during installation: | Notes from the sysadmin during installation: | ||
- | < | + | < |
- | $ wget https:// | + | $ wget https:// |
- | $ tar xf v2.0.3.tar.gz | + | $ unzip Trinity-v2.4.0.zip |
- | $ cd trinityrnaseq-2.0.3 | + | $ cd trinityrnaseq-Trinity-v2.4.0 |
- | $ sudo mkdir / | + | $ scl enable devtoolset-4 bash |
- | $ sudo chown aorth / | + | |
- | $ cp -r * / | + | |
- | $ scl enable devtoolset-2 bash | + | |
$ make | $ make | ||
$ make plugins | $ make plugins | ||
- | $ sudo chown -R root: | + | $ sudo mkdir / |
+ | $ make test_trinity | ||
+ | $ sudo cp -r . / |
trinity-software.txt · Last modified: 2020/10/28 11:28 by aorth