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trinity-software [2015/03/20 15:28] – [Usage] aorthtrinity-software [2015/03/20 15:32] – [Usage] aorth
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 <code>module avail trinity</code> <code>module avail trinity</code>
  
-**Note:** Trinity creates millions of small files -- which causes problems on the HPC storage server -- so you must direct output from these jobs to ///var/scratch// instead of your home folder.+**Note:** Trinity creates millions of small files -- which causes problems on the HPC storage server -- so you must direct output from these jobs to ///var/scratch// instead of your home folder. For example: 
 + 
 +<code>#!/bin/env bash 
 +#SBATCH -p highmem 
 +#SBATCH -J trinity 
 +#SBATCH -n 10 
 + 
 +module load trinity/v2.0.3 
 +module load bowtie/1.1.1 
 +module load samtools/1.2 
 + 
 +# cd to working dir just in case 
 +export WORKDIR=$SLURM_JOBID 
 +cd $WORKDIR 
 + 
 +# match CPUs to amount requested in SBATCH options! 
 +Trinity --seqType fq --max_memory 10G --left fish_R1.fastq --right fish_R2.fastq --CPU 10 --output trinity-fish2</code>
 ===== Installation ====== ===== Installation ======
 Notes from the sysadmin during installation: Notes from the sysadmin during installation:
trinity-software.txt · Last modified: 2020/10/28 11:28 by aorth