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qiime2-software [2018/05/21 06:59] aorthqiime2-software [2018/07/22 16:21] aorth
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 ====== QIIME2 ====== ====== QIIME2 ======
-QIIME™ (canonically pronounced chime) stands for Quantitative Insights Into Microbial Ecology. QIIME is an open-source bioinformatics pipeline for performing microbiome analysis from raw DNA sequencing data.+QIIME™ (canonically pronounced chime) stands for Quantitative Insights Into Microbial Ecology. QIIME is an open-source bioinformatics pipeline for performing microbiome analysis from raw DNA sequencing data. QIIME2 is a completely new and different version than QIIME1.
  
 ===== Information ===== ===== Information =====
  
-  * Version: 2018.4+  * Version: 2018.6
   * Added: May, 2018   * Added: May, 2018
 +  * Updated: July, 2018
   * Link: https://qiime2.org/   * Link: https://qiime2.org/
  
 ===== Usage ===== ===== Usage =====
  
-See versions of qiime2 which are available:+See versions of qiime2 that are available:
 <code>$ module avail qiime2</code> <code>$ module avail qiime2</code>
  
-Load a particular version:+Load a particular version into your environment and run it:
  
-<code>$ module load qiime2/2018.4</code>+<code>$ module load qiime2/2018.
 +$ qiime --help</code>
  
 ===== Installation ====== ===== Installation ======
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 $ bash Miniconda3-latest-Linux-x86_64.sh -p /export/apps/qiime2/conda $ bash Miniconda3-latest-Linux-x86_64.sh -p /export/apps/qiime2/conda
 $ source /export/apps/qiime2/conda/bin/activate $ source /export/apps/qiime2/conda/bin/activate
-$ wget https://data.qiime2.org/distro/core/qiime2-2018.4-py35-linux-conda.yml +$ conda update -n base conda 
-$ conda env create -p /export/apps/qiime2/2018.--file qiime2-2018.4-py35-linux-conda.yml +$ wget https://data.qiime2.org/distro/core/qiime2-2018.6-py35-linux-conda.yml 
-$ conda activate /export/apps/qiime2/2018.4+$ conda env create -p /export/apps/qiime2/2018.--file qiime2-2018.6-py35-linux-conda.yml 
 +$ conda activate /export/apps/qiime2/2018.6
 $ sudo chown -R root:root /export/apps/qiime2 $ sudo chown -R root:root /export/apps/qiime2
 $ qiime --help</code> $ qiime --help</code>
  
-Qiime2 has 240 dependencies and is extremely tricky to install without the use of [[https://conda.io/miniconda.html|Miniconda]]it is not even published on PyPy anymore, so installing it with pip is not an option. install Conda somewhere globally so that I can use it to install Qiime2. After the installation is done you don't technically *needConda itself, but Qiime2 will need Conda's Python so we must keep it around.+Qiime2 has 240 dependencies and is extremely tricky to install without the use of [[https://conda.io/miniconda.html|Miniconda]] (it is not even published on PyPy anymore, so installing it with pip is not an option). 
 + 
 +My strategy is to install Conda somewhere globally and then use it to install Qiime2. After the installation is done I think we don't technically need Conda itself anymore, but but I will keep it around for ease of upgrading and installing future versions. 
 + 
 +To create the [[https://github.com/ilri/hpc-environment-modules/tree/master/qiime2|modulefile]] I compared the output ''env'' before and after loading the Qiime2 environment with Conda.
qiime2-software.txt · Last modified: 2021/10/05 15:34 by aorth