genome_assemblers:mira
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| - | ====== MIRA ====== | ||
| - | MIRA is a whole genome shotgun and EST sequence assembler for Sanger, 454 and Solexa / Illumina data. | ||
| - | http:// | ||
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| - | MIRA 3 is able to perform true hybrid de-novo assemblies using reads gathered through Sanger, 454 or Solexa sequencing technologies. That is, it assembles reads instead of a mix of (eventually shredded) shredded consensus sequence and reads. The length of the Solexa sequences is not restricted, they can be 36mers to 150mers or more. | ||
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| - | An often used combination of current sequencing technologies is a mix of de-novo 454 assembly and Solexa mapping assemblies: 454 to get long contigs built, Solexa to get rid of the pesky 454 homopolymer problems. Here's the recipe I use for sequencing a bacterium de-novo and almost perfectly for comparatively little money: | ||
| - | * get your DNA sequenced at ~20x coverage with 454 FLX | ||
| - | * get the very same DNA sequenced at ~30-40x coverage with Solexa | ||
| - | * put the sequences of 454 and Solexa (and Sanger, if you have) into MIRA | ||
| - | * wait over night for the result | ||
| - | * add half a day or so for prettifying the resulting assembly and check remaining uncertainties (if you really want to) with gap4 | ||
genome_assemblers/mira.1277921088.txt.gz · Last modified: 2010/06/30 18:04 by 172.26.14.218