################################################################################ # # Copyright (c) 2010, Lawrence Livermore National Security, LLC. # Produced at the Lawrence Livermore National Laboratory # Written by Clinton Torres . # CODE-42036. # All rights reserved. # # This file is part of LAVA (LAMP Assay Versatile Analysis). For details, # see http://code.google.com/p/lava-dna/ . # Please also read the Additional BSD Notice. # # Redistribution and use in source and binary forms, with or without # modification, are permitted provided that the following conditions are met: # . Redistributions of source code must retain the above copyright notice, # this list of conditions and the disclaimer below. # . Redistributions in binary form must reproduce the above copyright notice, # this list of conditions and the disclaimer (as noted below) in the # documentation and/or other materials provided with the distribution. # . Neither the name of the LLNS/LLNL nor the names of its contributors may be # used to endorse or promote products derived from this software without # specific prior written permission. # # THIS SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS "AS IS" # AND ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE # IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE # ARE DISCLAIMED. IN NO EVENT SHALL LAWRENCE LIVERMORE NATIONAL SECURITY, LLC, # THE U.S. DEPARTMENT OF ENERGY OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT, # INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES # (INCLUDING, BUT NOT LIMITED TO, PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES; # LOSS OF USE, DATA, OR PROFITS; OR BUSINESS INTERRUPTION) HOWEVER CAUSED AND # ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT # (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF # THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE. # # Additional BSD Notice # 1. This notice is required to be provided under our contract with the # U.S. Department of Energy (DOE). This work was produced at Lawrence # Livermore National Laboratory under Contract No. DE-AC52-07NA27344 with # the DOE. # 2. Neither the United States Government nor Lawrence Livermore National # Security, LLC nor any of their employees, makes any warranty, express or # implied, or assumes any liability or responsibility for the accuracy, # completeness, or usefulness of any information, apparatus, product, or # process disclosed, or represents that its use would not infringe # privately-owned rights. # 3. Also, reference herein to any specific commercial products, process, or # services by trade name, trademark, manufacturer or otherwise does not # necessarily constitute or imply its endorsement, recommendation, or # favoring by the United States Government or Lawrence Livermore National # Security, LLC. The views and opinions of authors expressed herein do not # necessarily state or reflect those of the United States Government or # Lawrence Livermore National Security, LLC, and shall not be used for # advertising or product endorsement purposes. ################################################################################ package LLNL::LAVA::PrimerSet; use strict; use warnings; use Carp; use vars qw(@ISA); use LLNL::LAVA::Constants ":standard"; use LLNL::LAVA::Options ":standard"; use LLNL::LAVA::TagHolder; # is-a @ISA = ("LLNL::LAVA::TagHolder"); # POD-formatted documentation #------------------------------------------------------------------------------- =head1 NAME LLNL::LAVA::PrimerSet - A pair of LLNL::LAVA::PrimerInfo objects =head1 SYNOPSIS # Instantiation $pair = LLNL::LAVA::PrimerSet->new( { "start_location" => $startLocation, "end_location" => $endLocation, "length" => $length, }); # Member functions are named with "get" prefixes as an indicator that # they can't be used to modify the PCR pair, just to get info about it. $location = $pair->getStartLocation(); $location = $pair->getEndLocation(); $length = $pair->getLength(); # String in the form of "start-end". Depending on how you created the pair, # the end location might be a smaller number than the start location. It's # possible to have this behavior, we just don't explicitly support it yet. $rangeString = $pair->getRangeAsString(); # Tag use (inherited from TagHolder) $oligo->setTag("useful_statistic", $usefulStatistic); $exists = $oligo->getTagExists("useful_statistic"); $tm = $oligo->getTag("useful_statistic"); =head1 EXAMPLES See synopsis. =head1 DESCRIPTION PrimerSet is the object model of a group of oligos. Sub-classes implement behavior specific to different types of oligo combinations such as PCRPairs. =head1 APPENDIX The following documentation describes the functions in this package =cut #------------------------------------------------------------------------------- =head2 new Usage : $sigComponent = LLNL::LAVA::PrimerSet->new( { "start_location" => $startLocation, "end_location" => $endLocation, "length" => $length, # Redundant, but keeping for now. }); Function : Creates a new LLNL::LAVA::PrimerSet Arguments : Hash ref - Parameters for initialization including: start_location - Integer start location for the entire set end_location - Integer end location for the entire set length - Integer length/span of the entire set Example : See Usage Returns : A new LLNL::LAVA::PrimerSet =cut sub new { my ($classType, $paramHash_r) = @_; if(!defined $paramHash_r) { confess("programming error - first parameter is a required hash ref"); } if(ref($paramHash_r) ne "HASH") { confess("programming error - first parameter must be a hash reference"); } # Start should be a smaller number than end at this point my $startLocation = optionRequired($paramHash_r, "start_location"); my $endLocation = optionRequired($paramHash_r, "end_location"); my $length = optionRequired($paramHash_r, "length"); # Initialize via TagHolder my $this = $classType->SUPER::new({}); # Start location is the up-strand 5' location $this->{"d_startLocation"} = $startLocation; $this->{"d_endLocation"} = $endLocation; $this->{"d_length"} = $length; return $this; } #------------------------------------------------------------------------------- =head2 getStartLocation Usage : $location = $pair->getStartLocation(); Function : Get the 5' start location of the forward primer Arguments : Example : See Usage Returns : Integer - start location of the pair =cut sub getStartLocation { my ($this) = @_; return $this->{"d_startLocation"}; } #------------------------------------------------------------------------------- =head2 getEndLocation Usage : $location = $pair->getEndLocation(); Function : Get the 5' start location of the reverse primer, which is the 3' end location of the PCR pair! Arguments : Example : See Usage Returns : Integer - end location of the pair =cut sub getEndLocation { my ($this) = @_; return $this->{"d_endLocation"}; } #------------------------------------------------------------------------------- =head2 getLength Usage : $length = $pair->getLength(); Function : Get the lengh of this PCR pair, calculated from primer locations Arguments : Example : See Usage Returns : Integer - length of the expected PCR amplicon =cut sub getLength { my ($this) = @_; return $this->{"d_length"}; } #------------------------------------------------------------------------------- =head2 getRangeAsString Usage : $rangeString = $pair->getRangeAsString(); Function : Get a string in the form of "start-end" (e.g. "123-456"), where the two numbers depend on primer locations Arguments : Example : See Usage Returns : String - representation of the range of the primer pair =cut sub getRangeAsString { my ($this) = @_; my $rangeString = $this->{"d_startLocation"} . "-" . $this->{"d_endLocation"}; return $rangeString; } 1; # Lame! __END__ =head1 AUTHOR Clinton Torres (clinton.torres@llnl.gov) =head1 SEE ALSO =cut