The marginAlign package can be used to align reads to a reference genome and call single nucleotide variations (SNVs). It is specifically tailored for Oxford Nanopore Reads.
See versions of marginalign that are available:
$ module avail marginalign
Load one version into your environment and run it:
$ module load marginalign/git-2369dafd $ marginAlign
Notes from the sysadmin during installation:
$ cd /tmp $ git clone https://github.com/benedictpaten/marginAlign.git $ cd marginAlign $ git submodule update --init $ make $ sudo mkdir -p /export/apps/marginalign/git-2369dafd $ sudo chown aorth /export/apps/marginalign/git-2369dafd $ virtualenv --no-site-packages --distribute /export/apps/marginalign/git-2369dafd $ source /export/apps/marginalign/git-2369dafd/bin/activate $ pip install --upgrade setuptools pip $ pip install -r requirements.txt $ cp -r LICENSE.txt margin* README.md scripts src submodules /export/apps/marginalign/git-2369dafd $ sudo chown -R root:root /export/apps/marginalign/git-2369dafd