Table of Contents

mafft

MAFFT is a multiple sequence alignment program for unix-like operating systems. It offers a range of multiple alignment methods, L-INS-i (accurate; for alignment of <∼200 sequences), FFT-NS-2 (fast; for alignment of <∼30,000 sequences), etc.

Usage

Show all available versions:

$ module avail mafft

Load one version into your environment and run it:

$ module load mafft/7.505
$ mafft

Installation

Notes from the sysadmin during installation:

$ cd /tmp
$ wget https://mafft.cbrc.jp/alignment/software/mafft-7.505-with-extensions-src.tgz
$ tar xf mafft-7.505-with-extensions-src.tgz 
$ cd mafft-7.505-with-extensions/core
$ sed -i 's/PREFIX = \/usr\/local/PREFIX = \/export\/apps\/mafft\/7.505/' Makefile
$ make
$ sudo mkdir -p /export/apps/mafft/7.505
$ sudo chown aorth /export/apps/mafft/7.505
$ make install
$ cd ../extensions
$ vim Makefile
$ sed -i 's/PREFIX = \/usr\/local/PREFIX = \/export\/apps\/mafft\/7.505/' Makefile
$ make
$ make install
$ sudo chown -R root:root /export/apps/mafft/7.505