Table of Contents

IgBLAST

IgBLAST was developed at NCBI to facilitate analysis of immunoglobulin variable domain sequences but has recently been extended to perform analysis for T cell receptor (TR) sequences.

Information

Usage

See which versions of igblast are available:

$ module avail igblast

Load one version into your environment and run it:

$ module load igblast/1.10.0
$ igblastn
$ igblastp

Installation

Notes from the sysadmin during installation:

$ cd /tmp
$ wget ftp://ftp.ncbi.nih.gov/blast/executables/igblast/release/1.10.0/ncbi-igblast-1.10.0-x64-linux.tar.gz
$ tar xf ncbi-igblast-1.10.0-x64-linux.tar.gz
$ sudo mkdir -p /export/apps/igblast/1.10.0
$ sudo mkdir -p /export/apps/igblast/1.10.0/data
$ sudo cp -r ncbi-igblast-1.10.0/* /export/apps/igblast/1.10.0
$ sudo mkdir /export/apps/igblast/1.10.0/data
$ sudo chown aorth /export/apps/igblast/1.10.0/data
$ cd /export/apps/igblast/1.10.0/data
$ wget -r -nH --cut-dirs=4 ftp://ftp.ncbi.nih.gov/blast/executables/igblast/release/internal_data
$ wget -r -nH --cut-dirs=4 ftp://ftp.ncbi.nih.gov/blast/executables/igblast/release/database
$ wget -r -nH --cut-dirs=4 ftp://ftp.ncbi.nih.gov/blast/executables/igblast/release/optional_file
$ sudo chown -R root:root /export/apps/igblast/1.10.0/data