Table of Contents

FASTQ demux

A simple program to demultiplex Illumina FASTQ files based on barcodes in the FASTQ headers.

Information

Usage

See available versions:

$ module avail fastq_demux

Load one version into your environment and run it:

$ module load fastq_demux/git-dff5823f
$ fastq_demux

Installation

Notes from the sysadmin during installation:

$ module load python/3.10
$ sudo mkdir -p /export/apps/fastq_demux/git-dff5823f
$ sudo chown aorth /export/apps/fastq_demux/git-dff5823f
$ python -m venv /export/apps/fastq_demux/git-dff5823f/.venv
$ source /export/apps/fastq_demux/git-dff5823f/.venv/bin/activate
$ python -m pip install -U pip setuptools wheel
$ cd /var/scratch
$ git clone https://github.com/Molmed/fastq_demux
$ pip install -r requirements/prod .
$ sudo chown -R root:root /export/apps/fastq_demux/git-dff5823f