====== QIIME ====== QIIMEâ„¢ (canonically pronounced chime) stands for Quantitative Insights Into Microbial Ecology. QIIME is an open-source bioinformatics pipeline for performing microbiome analysis from raw DNA sequencing data. ===== Information ===== * Version: 1.9.1 * Added: March, 2017 * Link: http://qiime.org/ ===== Usage ===== See versions of qiime which are available: $ module avail qiime Load a particular version: $ module load qiime/1.9.1 ===== Installation ====== Notes from the sysadmin during installation: $ cd /tmp $ wget https://www.python.org/ftp/python/2.7.13/Python-2.7.13.tar.xz $ tar xf Python-2.7.13.tar.xz $ cd Python-2.7.13 $ scl enable devtoolset-2 bash $ ./configure --prefix=/export/apps/qiime/1.9.1 --enable-optimizations $ make -j4 $ sudo mkdir -p /export/apps/qiime/1.9.1 $ sudo chown -R aorth:aorth /export/apps/qiime/1.9.1 $ make install $ make maninstall $ exit (from devtoolset) $ wget https://bootstrap.pypa.io/get-pip.py -O /tmp/get-pip.py $ /export/apps/qiime/1.9.1/bin/python /tmp/get-pip.py $ /export/apps/qiime/1.9.1/bin/pip install numpy $ /export/apps/qiime/1.9.1/bin/pip install qiime # Fix problem with tkinter by changing the backend from TkAgg to Agg in: /export/apps/qiime/1.9.1/lib/python2.7/site-packages/matplotlib/mpl-data/matplotlibrc $ cp /export/apps/qiime/1.9.1/lib/python2.7/site-packages/qiime/support_files/qiime_config /export/apps/qiime/1.9.1/qiime_config $ print_qiime_config.py -t $ sudo chown -R root:root /export/apps/qiime/1.9.1 A dedicated Python installation is recommended so that QIIME can have complete control over the packages it needs to install without conflicting with the system's Python.