[[mkatari-bioinformatics-august-2013|Back to Manny's Bioinformatics Workshop Home]] ====== Processing Sam output for IGV ====== To see sam file in igv NOTE !! The index file MUST be in the same directory as the file. 1) load samtools module module load samtools 2) Create an index for the genome fasta file samtools faidx Mesculenta_147.fa 3) For each sam files, convert to bam file samtools view -S -b NDL06-132.sam > NDL06-132.bam 4) sort each bam file samtools sort NDL06-132.bam NDL06-132.bam.sorted 5) create index for each bam file samtools index NDL06-132.bam.sorted.bam