1. Install prerequisites [[http://gmod.org/wiki/GBrowse_2.0_Prerequisites]] sudo perl -MCPAN -e 'install Math::BigInt::Pari' sudo perl -MCPAN -e 'install Digest::SHA' sudo perl -MCPAN -e 'install Crypt::SSLeay' sudo perl -MCPAN -e 'install Net::OpenID::Consumer' sudo perl -MCPAN -e 'install Net::SMTP::SSL' sudo perl -MCPAN -e 'install Authen::SASL' 2.Install automatically using CPAN shell $sudo perl -MCPAN -e 'install Bio::Graphics::Browser2' . . . Directory for GBrowse's config and support files? [/etc/gbrowse2] Directory for GBrowse's static images & HTML files? [/var/www/html/gbrowse2] Directory for GBrowse's temporary data [/var/tmp/gbrowse2] Directory for GBrowse's sessions, uploaded tracks and other persistent data [/var/lib/gbrowse2] Directory for GBrowse's example databases [/var/lib/gbrowse2/databases] Directory for GBrowse's CGI script executables? [/var/www/cgi-bin/gb2] Internet port to run demo web site on (for demo)? [8000] Apache loadable module directory (for demo)? [/etc/httpd/modules] 3.Edit the following lines in /etc/gbrowse2/GBrowse.conf 65 upload_db_adaptor = DBI::mysql 66 upload_db_host = localhost 67 upload_db_user = gbrowse 68 upload_db_pass = secret 4. Create gbrowse user in mysql GRANT USAGE ON *.* TO 'gbrowse'@'localhost' IDENTIFIED BY PASSWORD 'secret' GRANT ALL PRIVILEGES ON `userdata\_%`.* TO 'gbrowse'@'localhost' GRANT ALL PRIVILEGES ON `gbrowse_login`.* TO 'gbrowse'@'localhost' GRANT ALL PRIVILEGES ON `gbrowse_login`.`gbrowse_login` TO 'gbrowse'@'localhost' 5. Create gbrowse user sudo -u apache gbrowse_create_account.pl gbrowse New password for gbrowse: New password for gbrowse (confirm): Enter user's full name (optional): Enter user's email address (optional): i.kahugu@cgiar.org Account "gbrowse": password successfully set. 6. Load Meta data to db /usr/bin/gbrowse_metadb_config.pl -admin gbrowse:secret 7. Set admin account gbrowse_set_admin_passwd.pl 8. Setup email forwarding **Edit the necessary lines on /etc/gbrowse2/GBrowse.conf** For now we are authenticating against gmail.com. Later we may use local mail server. 191 # here are some common options 192 # smtp_gateway = localhost # localhost has properly configured outgoing gateway 193 #smtp_gateway = 172.26.0.222 # indicated machine will forward email for you 194 smtp_gateway = smtp.gmail.com:465:ssl:gbrowsehpc:secret # use gmail with account "joe.user" and password "secret" 195 # smtp_gateway = none # disable outgoing email 196 #smtp_gateway = none # disable outgoing email 197 198 # These values are used in the login confirmation message sent during 199 # user registration. You may customize 200 application_name = GBrowse 201 application_name_long = The Generic Genome Browser 202 email_address = gbrowsehpc@gmail.com 9. Start gbrowse http://hpc.ilri.cgiar.org/gbrowse2/