====== augur ====== Augur is a bioinformatics toolkit to track evolution from sequence and serological data. It provides a collection of commands which are designed to be composable into larger processing pipelines. Augur originated as part of Nextstrain, an open-source project to harness the scientific and public health potential of pathogen genome data. ===== Information ===== * Version: 11.2.0 * Added: March, 2021 * Link: https://github.com/nextstrain/augur ===== Usage ===== See versions of augur which are available: $ module avail augur Load one version into your environment and run it: $ module load augur/11.2.0 $ augur To familiarize yourself with the Augur workflow you should try their sample ncov data, for example: $ interactive -c 4 -w compute05 -J nextstrain-augur $ git clone https://github.com/nextstrain/ncov.git /var/scratch/aorth-ncov $ cd /var/scratch/aorth-ncov $ module load augur/11.2.0 $ gzip -d -c data/example_sequences.fasta.gz > data/example_sequences.fasta $ snakemake --cores 4 --profile ./my_profiles/getting_started For more information see the [[https://docs.nextstrain.org/projects/augur/en/stable/|Augur documentation]]. ===== Installation ====== Notes from the sysadmin during installation: $ sudo mkdir -p /export/apps/augur/11.2.0 $ sudo chown aorth:aorth /export/apps/augur/11.2.0 $ module load python/3.7 $ python -m venv /export/apps/augur/11.2.0/.venv $ source /export/apps/augur/11.2.0/.venv/bin/activate $ pip install nextstrain-augur==11.2.0 $ pip install nextstrain-cli $ pip install snakemake $ module load mafft/7.313 RAxML/8.2.12 fasttree/2.1.11 iqtree/1.6.12 vcftools/0.1.16 nextalign/0.1.7 $ nextstrain setup-check $ sudo chown -R root:root /export/apps/augur/11.2.0