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mkatari-bioinformatics-august-2013-deseq [2015/06/17 09:06] mkatari |
mkatari-bioinformatics-august-2013-deseq [2015/08/21 14:13] (current) mkatari |
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| An important part of DESeq is to estimate dispersion. This is simply a form of variance for the genes. | An important part of DESeq is to estimate dispersion. This is simply a form of variance for the genes. | ||
| < | < | ||
| + | # if you have replicates do the following: | ||
| cds = estimateDispersions( cds ) | cds = estimateDispersions( cds ) | ||
| + | ### HOWEVER, If you have NO replicates, then try this | ||
| + | cds = estimateDispersions( cds, method=" | ||
| </ | </ | ||