====== samtools ====== samtools provide various utilities for manipulating alignments in the SAM format, including sorting, merging, indexing and generating alignments in a per-position format. * Latest Version: 1.11 * Added: February, 2015 * Updated: January, 2021 * Link: http://www.htslib.org ===== Usage ===== Show all available versions of samtools: $ module avail samtools Load a particular version of samtools into your environment and run it: $ module load samtools/1.11 $ samtools ===== Installation ===== Notes from the sysadmin during installation: $ cd /tmp $ wget https://github.com/samtools/samtools/releases/download/1.11/samtools-1.11.tar.bz2 $ tar xf samtools-1.11.tar.bz2 $ cd samtools-1.11 # install a newer compiler from Red Hat software collections $ sudo yum install devtoolset-7-gcc devtoolset-7-gcc-gfortran devtoolset-7-libquadmath-devel devtoolset-7-gcc-c++ $ scl enable devtoolset-7 bash $ ./configure --enable-plugins --enable-libcurl --prefix=/export/apps/samtools/1.11 --with-htslib=/export/apps/htslib/1.11 $ make $ sudo mkdir -p /export/apps/samtools/1.11 $ sudo chown aorth /export/apps/samtools/1.11 $ make install $ sudo chown -R root:root /export/apps/samtools/1.11 This links against htslib/1.11, which is installed as a module.